Biased 16S rDNA PCR amplification caused by interference from DNA flanking the template region
نویسندگان
چکیده
منابع مشابه
Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR.
The PCR is used widely for the study of rRNA genes amplified from mixed microbial populations. These studies resemble quantitative applications of PCR in that the templates are mixtures of homologs and the relative abundance of amplicons is thought to provide some measure of the gene ratios in the starting mixture. Although such studies have established the presence of novel rRNA genes in many ...
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Nocardiosis is an uncommon infection caused by the aerobic actinomycete nocardia. Identification of the pathogen is essential for the definitive diagnosis and for an effective treatment. This report describes a case of chronic granulomatous pleuritis caused by nocardia. A 59 year old Japanese man had a history of repeated pyothorax. Right pleural decortication and thoracic drainage were perform...
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 1998
ISSN: 0168-6496
DOI: 10.1016/s0168-6496(98)00031-2